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  Unexpected Artifact in Liver Biopsies - Page 4
Response from:
EO Hoffmann (ehoffm@lsumc.edu)
Date: Wednesday, July 02, 1997 08:58 AM

We had the same problem with stat renal transplant biopsies (1). The center of the needle biopsy is poorly fixed and does not take the toluidine blue due to poor OsO4 fixation. The problem may be avoided by microwave fixation (2&3) or just by fixation in warm fixative (1).

References:

  1. Flores TR, Hoffmann EO, Velasquez,M: Stat HRLM-TEM techniques for transplant renal biopsies. J Histotechnol. Submitted for publication June, 1997.
  2. Ted Pella, Inc: Microwave-assisted rapid tissue processing. Workshop booklet. P.O. Box 492477. Redding CA 96049, 1996.
  3. Giberson RT, Smith RL, Demaree RS: Three hour microwave tissue processing for transmission electron microscopy: From unfixed tissue to sections. Scanning 17, suppl. V:26-27, 1995.

Reply to: tflore@lsumc.edu

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