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Techniques and Technical Tips | |
| Unexpected Artifact in Liver Biopsies - Page 2 | ||
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To illustrate the artifact and to determine its extent within the specimen, we prepared several biopsies so we could cut epoxy cross sections of the specimen core for toluidine blue staining (Figure 1). We then cut thin sections that included the area of the block continuous from the outer edge through the center of the core for examination in the EM (Figure 2). This step allowed mapping of the specimen, and assured accurate correlation between sections taken from the same block for light and electron microscopy. In the micrographs below we illustrate the differences in ultrastructural morphology from the outside of the core to the center of the core (Figures 3 to 5). | |
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| Figure 1. Low magnification LM of biopsy core cross section, toluidine blue staining. | Figure 2. Higher magnification LM of core cross section to illustrate the difference in morphology from the periphery to the center. The hepatocytes in the center show significant shrinkage, which makes the sinusoids appear more prominent. | |
