COTM 1997. Case 1 - Light Microcopy

May 1, 1997

Electron Microscopy of Paraffin Embedded Tissue

Case 1 - Clinical History

Light Microscopy

The initial biopsy and the subsequent curettings from the lytic lesion in the mandible were light microscopically similar.

Fig 1. H and E
Original Mag 160X

A variety of inflammatory cells are admixed with pale staining cells.

Fig 2. H and E
Original Mag 400X

The Langerhan's cells (arrows) have grooved nuclei and pale staining eosinophilic cytoplasm. Inflammatory cells with dark nuclei consist of lymphocytes and rare plasma cells as well as eosinophils.

The pale staining cells with grooved nuclei (Fig 2) stained positive for S-100 protein.

Electron Microscopy

Case 2 - Clinical History

Case 3 - Clinical History

	Fig 1. H and E Original Mag 160X A variety of inflammatory cells are admixed with pale staining cells.
	Fig 2. H and E Original Mag 400X The Langerhan's cells (arrows) have grooved nuclei and pale staining eosinophilic cytoplasm. Inflammatory cells with dark nuclei consist of lymphocytes and rare plasma cells as well as eosinophils.